Development of the bioartificial vaginal wall: An in vitro stage

Objective. To obtain a tissue-engineered construct based on a nonwoven polycaprolactone carrier, human epithelial and stromal cells to make an artificial vagina. Material and methods. Fibrous material based on polycaprolactone was obtained by an electrospinning technique. A capillary method was used to colonize the full-thickness tissue-engineering design by stromal cells and a static method was employed to colonize the surface layer of the construct by vaginal epithelial cells. Results. The vaginal epithelial cells expressed EpSAM and p63; the stromal cells did vimentin and smooth muscle actin. After colonization, the stromal cells were evenly distributed in a 1.5-mm thick matrix; the epithelial cells were arranged in a dense layer on the inner surface of the construct, sinking to a depth of 88.9±32.5 μm. Conclusion. The tissue-engineered construct obtained is similar to the native vagina in architectonics and cell composition. © Bionika Media Ltd.

Arutyunyan I.V. 1, 2 , Tenchurin T.K.3 , Grigoryev T.E.3 , Makarov A.V. 1, 4 , Korshunov A.A.1 , Elchaninov A.V. 1, 2 , Kananykhina Y.Y.1, 2 , Lokhonina A.V. 1 , Vasyukova O.A.1, 2 , Uvarova E.V.1 , Chuprynin V.D.1 , Shepelev A.D.3 , Mamagulashvili V.G.3 , Chvalun S.N.3 , Fatkhudinov T.K. 1, 5
ООО «Бионика Медиа»
Номер выпуска
  • 1 National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia, Ac. Oparina str. 4, Moscow, 117997, Russian Federation
  • 2 Research Institute of Human Morphology, Ac. Oparina str. 4, Moscow, 117997, Russian Federation
  • 3 Kurchatov Complex of Nano-, Bio-, Information, Cognitive, and Sociohumanistic Technologies, National Research Center, Kurchatov Institute, Academician Kurchatov sq. 1, Moscow, 123182, Russian Federation
  • 4 Department of Histology, N.I. Pirogov Russian National Research Medical University, Ac. Oparina str. 4, Moscow, 117997, Russian Federation
  • 5 Department of Histology, Cytology and Embryology of Peoples’ Friendship University of Russia, Ac. Oparina str. 4., Moscow, 117997, Russian Federation
Ключевые слова
Electrospinning; Multipotent stromal cells; Polycaprolactone matrix; Tissue engineering; Vagina; Vaginal epithelium
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