Activated Macrophages of Monocytic Origin Predominantly Express Proinflammatory Cytokine Genes, whereas Kupffer Cells Predominantly Express Anti-Inflammatory Cytokine Genes

In the central nervous system and in the liver, the macrophage populations are represented exclusively by descendants of the hematopoietic progenitor cells of the yolk sac. The reasons for such differential distribution of macrophages are not fully understood. We found that, as can be judged by corresponding changes in the expression of CD86 and CD163 markers, the transient macrophages of monocytic lineage are more sensitive to activating stimuli. The two macrophage populations have distinct patterns of gene expression, which is particularly noticeable for M1- and M2-associated genes. For instance, Kupffer cells more readily develop and longer maintain the elevated expression levels of Il4, Il10, and Il13 upon the activation; by contrast, the macrophages of monocytic lineage express Il1b, Il12a, and Tnfα upon the activation. The obtained results allow us to conclude that the in vitro activated Kupffer cells of the liver are committed to M2 phenotype, whereas the in vitro activated monocyte-derived macrophages show a typical M1 behavior. These observations are likely to reflect the situation in the in vivo microenvironments. © 2019 Anastasia Lokhonina et al.

Lokhonina A. 1 , Elchaninov A. 1, 2 , Fatkhudinov T. 1, 3 , Makarov A. 1, 2 , Arutyunyan I. 1, 4 , Grinberg M. 3 , Glinkina V.2 , Surovtsev V. 3 , Bolshakova G.4 , Goldshtein D.5 , Sukhikh G.1
Hindawi Limited
  • 1 National Medical Research Center for Obstetrics, Gynecol. and Perinatology Named after Academician V.I. Kulakov of Min. of Hlthcare. of Russ. Fed., 4 Oparina Street, Moscow, 117997, Russian Federation
  • 2 Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, 1 Ostrovitianov Street, Moscow, 117997, Russian Federation
  • 3 Peoples' Friendship University of Russia, 6 Miklukho-Maklaya Street, Moscow, 117198, Russian Federation
  • 4 Scientific Research Institute of Human Morphology, 3 Tsurupa Street, Moscow, 117418, Russian Federation
  • 5 Research Centre of Medical Genetics, 1 Moscvorechie, Moscow, 115478, Russian Federation
Ключевые слова
CD163 antigen; CD86 antigen; cytokine; interleukin 1; interleukin 10; interleukin 12; interleukin 13; interleukin 18; interleukin 4; interleukin 6; tumor necrosis factor; animal experiment; Article; cell differentiation; cell lineage; cell permeabilization; comparative study; controlled study; cytokine production; enzyme linked immunosorbent assay; flow cytometry; gene expression; in vitro study; Kupffer cell; macrophage activation; male; monocyte; nonhuman; nucleotide sequence; phagocytosis; phenotype; rat; stem cell; upregulation; yolk sac
Дата создания
Дата изменения
Постоянная ссылка

Другие записи