Albumin-Functionalized Iron Oxide Nanoparticles for Theranostics: Engineering and Long-Term In Situ Imaging

Magnetic nanosystems (MNSs) consisting of magnetic iron oxide nanoparticles (IONPs) coated by human serum albumin (HSA), commonly used as a component of hybrid nanosystems for theranostics, were engineered and characterized. The HSA coating was obtained by means of adsorption and free radical modification of the protein molecules on the surface of IONPs exhibiting peroxidase-like activity. The generation of hydroxyl radicals in the reaction of IONPs with hydrogen peroxide was proven by the spin trap technique. The methods of dynamic light scattering (DLS) and electron magnetic resonance (EMR) were applied to confirm the stability of the coatings formed on the surface of the IONPs. The synthesized MNSs (d ~35 nm by DLS) were intraarterially administered in tumors implanted to rats in the dose range from 20 to 60 μg per animal and studied in vivo as a contrasting agent for computed tomography. The long-term (within 14 days of the experiment) presence of the MNSs in the tumor vascular bed was detected without immediate or delayed adverse reactions and significant systemic toxic effects during the observation period. The peroxidase-like activity of MNSs was proven by the colorimetric test with o-phenylenediamine (OPD) as a substrate. The potential of the synthesized MNSs to be used for theranostics, particularly, in oncology, was discussed.

Bychkova Anna V.1 , Yakunina Marina N.2 , Lopukhova Mariia V.1 , Degtyarev Yevgeniy N.1, 3 , Motyakin Mikhail V.1, 3 , Pokrovsky Vadim S. 1, 2, 4, 5 , Kovarski Alexander L.1 , Gorobets Maria G.1 , Retivov Vasily M.6, 7 , Khachatryan Derenik S.6, 7
Номер выпуска
  • 1 Emanuel Institute of Biochemical Physics of Russian Academy of Sciences
  • 2 N.N. Blokhin National Medical Research Center of Oncology
  • 3 N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences
  • 4 Laboratory of Experimental Oncology, Research Institute of Molecular and Cellular Medicine, RUDN University
  • 5 Department of Biotechnology, Sirius University of Science and Technology
  • 6 The Federal State Unitary Enterprise, Institute of Chemical Reagents and High Purity Chemical Substances of National Research Center “Kurchatov Institute”
  • 7 National Research Center “Kurchatov Institute”
Ключевые слова
human serum albumin (HSA); iron oxide magnetic nanoparticles with peroxidase-like activity; free radical approach (FRA); computed tomography (CT); theranostics
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Другие записи

Machulkin Aleksei E., Nimenko Ekaterina A., Zyk Nikolay U., Uspenskaia Anastasiia A., Smirnova Galina B., Khan Irina I., Pokrovsky Vadim S., Vaneev Alexander N., Timoshenko Roman V., Mamed-Nabizade Vugara V., Zavertkina Maria V., Erofeev Alexander, Gorelkin Petr, Majouga Alexander G., Zyk Nikolay V., Khazanova Elena S., Beloglazkina Elena K.
Molecules. MDPI AG. Том 27. 2022. 8795 с.