Early-changes diagnostics of erythrocytes microrheological features in the model of dyslipidemia development in rats at the late stages of ontogenesis
In our work we traced the dependence of erythrocyte microrheological features dynamics on the age of experimentally developed dislipidemia in rats. The investigation was fulfilled with the help of initially healthy rats of Vistar line. The experimental group was composed of 105 male rats (34 rats, aged 12 months; 32 rats, aged 18 months; 39 rats, aged 24 months) which were exposed to dislipidemia development by alimentary way. The control group was composed of 91 male rats, including 30 rats aged 12 months, 32 rats aged 18 months, and 29 rats aged 24 months. We applied biochemical, hematological, and statistical methods of investigation. Experimental dislipidemia development in rats showed decrease of plasma antioxidant protection and increase of lipids' peroxidation, which deepened with aging of animals taken into the experiment. With dislipidemia development in experimental rats, we noticed that during the aging process there was a rapid rise in the number of erythrocytes that both reversibly and irreversibly lost their biconcave form. In the experimental group of animals, we noticed during the aging process an increase in erythrocytes' aggregation. Development of abnormalities in erythrocytes' microrheological features in experimental rats exceeded the aging-related changes in the control group. Changes thus found contributed significantly to aggravation in morbidity and heightened sensitivity of animals to negative impacts of environmental factors. Therefore, during the aging process, it is necessary to control more and more strictly the level of blood lipids in order to avoid the development of dislipidemia which becomes acutely dangerous especially during aging because of worsening hemocirculation and rise in the risk of thrombophilia development.