Bio-remediation capacity for Cd(II) and Pb(II) from the aqueous medium by two novel strains of microalgae and their effect on lipidomics and metabolomics

Microalgae have been the subject of recent research as a sustainable feedstock for the large-scale production of metabolites for commercial purposes. This study presents a green bio-remediation approach towards heavy metal contaminations and biomass production for biofuels in microalgae metabolomics and lipidomics approaches. Two novel microalgae, Chlorosarcinopsis bastropiensis and Polyedriopsis spinulosa, were isolated during the study and subjected to Pb(II) and Cd(II) pollutants. The isolated microalgae strains have shown a varied behavior towards cell growth, pigment accumulation, and lipids profiles during the impact of short-term (96 h) and long-term (14 d) heavy metal tolerance. Cell viability and IC50 value (397.75 mg/L for C. bastropiensis and 490.16 mg/L for P. spinulosa) have indicated higher tolerance towards Pb(II) in both microalgae. FTIR analysis of microalgal biomass has revealed insignificant differences during long and short-term heavy metal toxicity, clearly indicating the bio-tolerance for Pb(II) and Cd(II) in both microalgae. Principal component analysis has revealed the expression of metabolites (such as glycine, proline, valine, isoleucine, linoleic acid, glucose, sucrose, etc.) under heavy metal stress. 1H NMR analysis has demonstrated the prominent expression of metabolites under heavy metal stress. ICP-MS-based studies do not reflect the correlation between cellular tolerance and bioaccumulation of each heavy metal by both microalgae. Lipidomics based on 1H NMR has revealed an increase in unsaturated fatty acids under the impact of heavy metals. Therefore, this study offers a sustainable bioremediation technique for heavy metal contaminants and biomass production with significant enhancement of metabolites and lipid components for biofuels and/-or other commercial applications. © 2021 Elsevier Ltd

Авторы
Nanda M.1 , Jaiswal K.K.2 , Kumar V. 3, 4 , Verma M.5 , Vlaskin M.S.6 , Gururani P.7 , Kim H. 5 , Alajmi M.F.8 , Hussain A.8
Издательство
Elsevier Ltd
Язык
Английский
Статус
Опубликовано
Номер
102404
Том
44
Год
2021
Организации
  • 1 Department of Biotechnology, Dolphin (P.G.) Institute of Biomedical and Natural Sciences, Dehradun, 248007, India
  • 2 Institute for Water and Wastewater Technology, Durban University of Technology, Durban, 4000, South Africa
  • 3 Department of Life Sciences, Graphic Era (Deemed to Be University), Dehradun, Uttarakhand 248002, India
  • 4 Peoples' Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
  • 5 Department of Environmental Engineering, University of Seoul, Seoul, 130743, South Korea
  • 6 Joint Institute for High Temperatures of the Russian Academy of Sciences, 13/2 Izhorskaya St, Moscow, 125412, Russian Federation
  • 7 Department of Biotechnology, Graphic Era (Deemed to Be University), Dehradun, Uttarakhand 248002, India
  • 8 Department of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia
Ключевые слова
Heavy metal tolerance, bio-remediation; Lipidomics; Metabolomics; Microalgae
Дата создания
16.12.2021
Дата изменения
16.12.2021
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/76454/
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