Micro-pollutant Pb(II) mitigation and lipid induction in oleaginous microalgae Chlorella sorokiniana UUIND6

Green oleaginous microalgae are good candidates for the integration of heavy metal bioremediation with the generation of biofuels. To be effective, this requires a comprehensive understanding and optimization of the metal concentration that generates maximum growth along with a high yield of lipids from microalgae cells. This work follows the aforementioned strategy and involves the use of oleaginous microalgae Chlorella sorokiniana for lead mitigation and biodiesel production. The short-term effects (IC50 value at 96 h of culture) and the long-term effects (pigments and biomass) of the exposure of lead (Pb) to the growth and biochemical compositions (pigments, proteins, carbohydrates, and lipids) of microalgae have been also determined. Chlorella sorokiniana has a high half-maximal inhibitory concentration (IC50 value and higher metal bio-concentration factor (BCF) for Pb (II), which represents that this strain can be considered as a lead (Pb) hyperbioaccumulator. FTIR analysis revealed a reduction in proteins and carbohydrates under the influence of Pb while an increase in lipids has been recorded. The lipid profile based on 1H NMR and GC–MS has been followed for the quality analysis of the biodiesel produced that indicated altered lipid profiles under stress by Pb and elevated levels of SFA and MUFA. © 2021 Elsevier B.V.

Nanda M.1 , Jaiswal K.K.2, 3 , Kumar V. 4, 5 , Vlaskin M.S.6 , Gautam P.4 , Bahuguna V.7 , Chauhan P.K.8
Elsevier B.V.
  • 1 Department of Biotechnology, Dolphin (PG) Institute of Biomedical & Natural Sciences, Dehradun, Uttarakhand 248007, India
  • 2 Algae Research and Bio-energy Laboratory, Department of Chemistry, Uttaranchal University, Dehradun, Uttarakhand 248007, India
  • 3 Institute for Water and Wastewater Technology, Durban University of Technology, Durban, 4000, South Africa
  • 4 Department of Life Sciences, Graphic Era (Deemed to Be University), Dehradun, Uttarakhand 248002, India
  • 5 Peoples’ Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
  • 6 Joint Institute for High Temperatures of the Russian Academy of Sciences, 13/2 Izhorskaya St, Moscow, 125412, Russian Federation
  • 7 Department of Biotechnology, Uttaranchal University, Dehradun, Uttarakhand 248007, India
  • 8 Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, Himachal Pradesh 173229, India
Ключевые слова
Biodiesel; ICPMS; Lead; Lipid accumulation; Microalgae; Pb bioremediation
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Другие записи

Vishnyakova P., Poltavets A., Karpulevich E., Maznina A., Vtorushina V., Mikhaleva L., Kananykhina E., Lokhonina A., Kovalchuk S., Makarov A., Elchaninov A., Sukhikh G., Fatkhudinov T.
Biomedicine and Pharmacotherapy. Elsevier Masson SAS. Том 139. 2021.