Macroheterocycles containing aza-14-crown-4 ether subunit were synthesized by one-step domino reaction. They are interesting objects for study of physicochemical properties and types of transformation. The N-acylated and N-alkylated aza-14-crown-4 ethers were obtained in good yields. The new hybrid compound - bis(furanyl)triazinethiolazacrown ether - was formed by S-alkylation of N-chloroacyl derivative (3). According to the PASS program, these substances can be inhibitors of the permeability of cell membrane and act also as a CYP2H substrate. © ISUCT Publishing.