Investigation of the complex antibiotic INA-5812

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study. © 2016, Pleiades Publishing, Ltd.

Lapchinskaya O.A.1 , Katrukha G.S.1 , Gladkikh E.G.1 , Kulyaeva V.V.1 , Coodan P.V.2 , Topolyan A.P.3 , Alferova V.A.1 , Pogozheva V.V.1 , Sukonnikov M.A.4 , Rogozhin E.A.1, 3 , Prokhorenko I.A.1, 3 , Brylev V.A.3 , Korolev A.M.1 , Slyundina M.S.1, 5 , Borisov R.S. 5, 6 , Serebryakova M.V.7 , Shuvalov M.V.1, 4 , Ksenofontov A.L.7 , Stoyanova L.G.8 , Osterman I.A.4, 7 , Formanovsky A.A.3 , Tashlitsky V.N.4 , Baratova L.A.7 , Timofeeva A.V.7 , Tyurin A.P.1, 3
Номер выпуска
  • 1 Gause Institute of New Antibiotics, Moscow, 119021, Russian Federation
  • 2 Federal Research Center of Nutrition and Biotechnology, Moscow, 109240, Russian Federation
  • 3 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russian Federation
  • 4 Faculty of Chemistry, Moscow State University, Moscow, 119991, Russian Federation
  • 5 Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Moscow, 119991, Russian Federation
  • 6 Faculty of Physics, Mathematics and Natural Sciences, Russian University of Peoples’ Friendship, Moscow, 115419, Russian Federation
  • 7 Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russian Federation
  • 8 Faculty of Biology, Moscow State University, Moscow, 119991, Russian Federation
Ключевые слова
glycopeptide antibiotics; high performance liquid chromatography; high-resolution mass spectrometry; microbial metabolites
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