β-endorphin-like peptide SLTCLVKGFY is a selective agonist of nonopioid β-endorphin receptor

It has been found that β-endorphin (β-END) and a synthetic β-END-like decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (termed immunorphin, IMN) corresponding to the sequence 364-373 of human IgG heavy chain stimulate Con A-induced proliferation of T lymphocytes from the blood of healthy donors. [Met5]enkephalin ([Met5]ENK) and an antagonist of opioid receptors naloxone (NAL) tested in parallel were not active. The stimulating effect of β-END and IMN on T lymphocyte proliferation was not inhibited by NAL. Studies on receptor binding of 125I-labeled IMN to T lymphocytes revealed that it binds with high affinity to NAL-insensitive binding sites (Kd = 7.0 ± 0.3 nM). Unlabeled β-END completely inhibited the specific binding of 125I-labeled IMN to NAL-insensitive binding sites on T lymphocytes (Ki = 1.1 ± 0.2 nM). Thus, β-END and IMN bind to common NAL-insensitive binding sites on T lymphocytes and enhance Con A-induced proliferation of these cells. © 2002 Elsevier Science (USA).

Авторы
Navolotskaya E.V.1 , Zargarova T.A.1 , Malkova N.V.1 , Krasnova S.B.1 , Lipkin V.M.1 , Zav’yalov V.P. 2
Редакторы
-
Издательство
-
Номер выпуска
4
Язык
Английский
Страницы
799-804
Статус
Опубликовано
Подразделение
-
Номер
-
Том
292
Год
2002
Организации
  • 1 Branch of Shemyakin, Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Science Avenue, 6, 142290, Pushchino, Moscow Region, Russian Federation
  • 2 Department of Biomedical Technology, Medical Faculty, Russian University of Friendship of Peoples, Miklukho-Maklaya Street, 6117198, Moscow, Russian Federation
Ключевые слова
Immunoglobulin G; Naloxone-insensitive binding site; T lymphocyte; β-endorphin
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/251/