Synthetic peptide SLTCLVKGFY competes with β-endorphin for naloxone-insensitive binding sites on rat brain membranes

The synthetic decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (termed immunorphin) corresponding to the sequence 364-373 of the CH3 domain of human immunoglobulin G heavy chain and its synthetic fragment VKGFY were found to compete with 125I-labeled β-endorphin for high-affinity naloxone-insensitive binding sites on membranes isolated from the rat brain cortex (Ki=1.18±0.09 and 1.58±0.11nM, respectively). The binding specificity study revealed that these binding sites were insensitive not only to naloxone but to [Met5]enkephalin and [Leu5]enkephalin as well. The Kd values characterizing the specific binding of 125I-labeled immunorphin and its fragment Val-Lys-Gly-Phe-Tyr to these binding sites were determined to be 2.93±0.27nM and 3.17±0.29nM, respectively. © 2002 Elsevier Science Inc. All rights reserved.

Авторы
Navolotskaya E.V.1 , Zargarova T.A.1 , Malkova N.V.1 , Krasnova S.B.1 , Zav'yalov V.P. 2 , Lipkin V.M.1
Редакторы
-
Журнал
Издательство
-
Номер выпуска
6
Язык
Английский
Страницы
1115-1119
Статус
Опубликовано
Подразделение
-
Номер
-
Том
23
Год
2002
Организации
  • 1 Branch of Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation
  • 2 Department of Biomedical Technology, Medical Faculty, Russian University of Friendship of Peoples, ul. Miklukho-Maklaya 6, 117198 Moscow, Russian Federation
Ключевые слова
β-Endorphin; Peptide; Receptor; T lymphocytes
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/184/