Toxicity Evaluation and Controlled-Release of Curcumin-Loaded Amphiphilic Poly-N-vinylpyrrolidone Nanoparticles: In Vitro and In Vivo Models

Curcumin attracts huge attention because of its biological properties: it is antiproliferative, antioxidant, anti-inflammatory, immunomodulatory and so on. However, its usage has been limited by poor water solubility and low bioavailability. Herein, to solve these problems, we developed curcumin-loaded nanoparticles based on end-capped amphiphilic poly(N-vinylpyrrolidone). Nanoparticles were obtained using the solvent evaporation method and were characterized by dynamic and electrophoretic light scattering, transmission electron (TEM) and atomic force (AFM) microscopy. The average particle size was 200 nm, and the ζ-potential was −4 mV. Curcumin-release studies showed that nanoparticles are stable in aqueous solutions. An in vitro release study showed prolonged action in gastric, intestinal and colonic fluids, consistently, and in PBS. In vitro studies on epidermoid carcinoma and human embryonic kidney cells showed that the cells absorbed more curcumin in nanoparticles compared to free curcumin. Nanoparticles are safe for healthy cells and show high cytotoxicity for glioblastoma cells in cytotoxicity studies in vitro. The median lethal dose was determined in an acute toxicity assay on zebrafish and was 23 μM. Overall, the curcumin-loaded nanoparticles seem promising for cancer treatment.

Авторы
Luss Anna L.1 , Bagrov Dmitry V.2 , Yagolovich Anne V.2 , Kukovyakina Ekaterina V.1 , Khan Irina I. 3, 4 , Pokrovsky Vadim S. 3, 4 , Shestovskaya Maria V.1 , Gasparian Marine E.1, 5 , Dolgikh Dmitry A.2, 5 , Kuskov Andrey N.1
Журнал
Издательство
MDPI AG
Номер выпуска
1
Язык
Английский
Страницы
8
Статус
Опубликовано
Том
16
Год
2024
Организации
  • 1 Department of Technology of Chemical, Pharmaceutical and Cosmetic Substances, D. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia
  • 2 Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia
  • 3 N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, 115478 Moscow, Russia
  • 4 Department of Biochemistry, People’s Friendship University of Russia (RUDN University), 117198 Moscow, Russia
  • 5 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia
Дата создания
28.01.2024
Дата изменения
28.01.2024
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/106153/
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