Conjugation of L-lysine A-oxidase with antibodies

The conjugation of the drugs with vector molecules enables to obtain therapeutic preparation, which may be transported to the selected target organ. In the present work the methods of conjugation of antineoplastic enzyme L-lysine α-oxidase with antibodies were elaborated. Conjugates were worked out through the attachment of amino groups on the antibody surface either with the aldehyde groups which were created in L-lysine α-oxidase molecule (0.2% of initial enzymatic activity) or with the aldehyde groups of cross-linking molecules. Maximal (78%) L-lysine α-oxidase activity in conjugates was observed when oxidized peroxidase which contained the aldehyde, groups was used as crosslinking agent. The glutaraldehyde method yielded 70% of initial enzyme activity.

Authors
Gogichaeva N.V. 1 , Lukasheva E.V. 1 , Gavrilova E.M.2 , Smirnova I.P. 1 , Egorov A.M.2 , Berezov T.T. 1
Editors
-
Publisher
-
Number of issue
4
Language
Russian
Pages
417-418
Status
Published
Department
-
DOI
-
Number
-
Volume
46
Year
2000
Organizations
  • 1 People's Friendship University, Moscow, Russian Federation
  • 2 Moscow State Lomonosov University, Russian Federation
Keywords
Antibodies; Conjugate; Directed transport; L-lysine α-oxidase; Peroxidase
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/464/