Composite glycerol/graphite/aromatic acid matrices for thin-layer chromatography/matrix-assisted laser desorption/ionization mass spectrometry of heterocyclic compounds

New composite matrices have been suggested for the analysis of mixtures of different synthetic organic compounds (N-containing heterocycles and erectile dysfunction drugs) by thin layer chromatography/matrix-assisted laser desorption ionization time-of-flight mass spectrometry (TLC/MALDI-TOF). Different mixtures of classical MALDI matrices and graphite particles dispersed in glycerol were used for the registration of MALDI mass spectra directly from TLC plates after analytes separation. In most of cases, the mass spectra possessed [M+H]+ ions; however, for some analytes only [M+Na]+ and [M+K]+ ions were observed. These ions have been used to generate visualized TLC chromatograms. The described approach increases the desorption/ionization efficiencies of analytes separated by TLC, prevent spot blurring, simplifies and decrease time for sample preparation. © 2016 Elsevier B.V.

Authors
Esparza C.1 , Borisov R.S. 1, 2 , Varlamov A.V. 1 , Zaikin V.G. 2
Language
English
Pages
118-122
Status
Published
Volume
1470
Year
2016
Organizations
  • 1 People Friendship University of Russia, Mikluho-maklaya st. 6, Moscow, Russian Federation
  • 2 A.V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninskii pr. 29, Moscow, 119991, Russian Federation
Keywords
Composite matrix; Graphite; Heterocycles; MALDI; Mass spectrometry; Sildenafil; TLC; Vardenafil
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/3770/