Technologies for Viable Circulating Tumor Cell Isolation

The spread of tumor cells throughout the body by traveling through the bloodstream is a critical step in metastasis, which continues to be the main cause of cancer-related death. The detection and analysis of circulating tumor cells (CTCs) is important for understanding the biology of metastasis and the development of antimetastatic therapy. However, the isolation of CTCs is challenging due to their high heterogeneity and low representation in the bloodstream. Different isolation methods have been suggested, but most of them lead to CTC damage. However, viable CTCs are an effective source for developing preclinical models to perform drug screening and model the metastatic cascade. In this review, we summarize the available literature on methods for isolating viable CTCs based on different properties of cells. Particular attention is paid to the importance of in vitro and in vivo models obtained from CTCs. Finally, we emphasize the current limitations in CTC isolation and suggest potential solutions to overcome them.

Авторы
Tretyakova Maria S.1 , Menyailo Maxim E. 2 , Schegoleva Anastasia A. 1, 2 , Bokova Ustinia A.1 , Larionova Irina V.1 , Denisov Evgeny V. 1, 2
Издательство
MDPI AG
Номер выпуска
24
Язык
Английский
Страницы
15979
Статус
Опубликовано
Том
23
Год
2022
Организации
  • 1 Laboratory of Cancer Progression Biology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia
  • 2 Single Cell Biology Laboratory, Research Institute of Molecular and Cellular Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, Russia
Ключевые слова
CTC; isolation; cancer; metastasis; in vitro; in vivo
Дата создания
17.10.2023
Дата изменения
17.10.2023
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/94371/
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