Amyloid-beta induced cell death is independent of free radicals

Acutely dissociated rat cerebellar granule cell neurons were incubated with amyloid-beta (1-42) and studied by flow cytometry. Amyloid-beta caused a dose-dependent loss of viability, as determined by intracellular accumulation of propidium iodide (PI),and that was not accompanied by significant elevation of intracellular calcium, measured by Fluo-3 or reactive oxygen species (ROS), measured by 2,7'-dihydro-dichlorfluorescein diacetate (DCF). Carnosine, a ROS scavenger and an inhibitor of non-enzymatic glycosylation, partially reduced cell death caused by amyloid-beta. We conclude that amyloid-beta causes a relatively acute loss of cell viability in cerebellar granule cell neurons, which does not result from either elevation of intracellular calcium concentration or generation of ROS.

Авторы
Boldyrev A. , Koudinov A. , Berezov T. , Carpenter D.O.
Редакторы
-
Издательство
IOS Press
Номер выпуска
6
Язык
Английский
Страницы
633-638
Статус
Опубликовано
Подразделение
-
Ссылка
-
DOI
-
Номер
-
Том
6
Год
2004
Организации
-
Ключевые слова
flow cytometry; reactive oxygen species; calcium; carnosine; cerebellum; granule cells; Alzheimer's disease
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/8872/