High rate of mutational load in case of bladder cancer (BC), surpassed in this regard only by melanoma and lung cancer, determines tumor sensitivity to immunotherapeutic treatment methods and makes it one of the most promising targets for immunotherapy. Inhibitors of check-points such as CTLA-4, PD-1 and PD-L1 and antitumor vaccines have already proved their activity in case of these diseases. Supposedly, the very significant amount of mutations in the tumor, having high level of expression of tumor-associated antigens for their presentation in immune system, is the important factor of efficiency of immunodrugs. Development of personified dendritic cell antitumor vaccines against BC poses an urgent problem, which covers many aspects, necessary for its standardization. Particularly, in case of cultivation of tumor cells under in vitro conditions their transformation goes at higher pace in comparison with in vivo tumor development, which must be considered when selecting cell lines for research. The work presents details related to research of cytogenetic peculiarities of BC tumor cultures in case of long-term cultivation and detection of correlation between cytogenetic profile and expression of tumor-specific cancer-testis antigens.