Bladder cancer (BC) in patients is characterized by different extent of invasion and malignancy. Inhomogeneity of immunological and biological markers, moderate level of evidence of their clinical and diagnostic significance requires performance of further studies for research of new and discovery of optimal combinations of existing immunobiological BC markers and establishment of possible differences. The most highly immunogenic antigens are cancer-testis antigens (CTA), expression of which in case of different forms of BC is poorly explored. The work contains comparative data from research of CTA (MAGE, BAGE, NY-ESO-1, GAGE) expression in case of invasive and non-invasive forms of BC. Difference of CTA expression profile depending on the form of BC invasion is demonstrated, which opens opportunities for their use for creation of anti-tumor vaccines in case of BC.