Targeted Lipidomic Analysis of Aqueous Humor Reveals Signaling Lipid-Mediated Pathways in Primary Open-Angle Glaucoma

Simple Summary Analysis of the eye liquids collected from a cohort of primary open-angle glaucoma patients identified signaling lipids, the pattern of which suggests a role of arachidonic acid/platelet activating-factor (PAF)-dependent pathways and oxidative stress in the pathogenesis of the disease and provides novel targets for its diagnostics and treatment. Primary open-angle glaucoma (POAG) is characterized by degeneration of retinal ganglion cells associated with an increase in intraocular pressure (IOP) due to hindered aqueous humor (AH) drainage through the trabecular meshwork and uveoscleral pathway. Polyunsaturated fatty acids and oxylipins are signaling lipids regulating neuroinflammation, neuronal survival and AH outflow. Among them, prostaglandins have been previously implicated in glaucoma and employed for its treatment. This study addressed the role of signaling lipids in glaucoma by determining their changes in AH accompanying IOP growth and progression of the disease. Eye liquids were collected from patients with POAG of different stages and cataract patients without glaucoma. Lipids were identified and quantified by UPLC-MS/MS. The compounds discriminating glaucoma groups were recognized using ANCOVA and PLS-DA statistic approaches and their biosynthetic pathways were predicted by bioinformatics. Among 22 signaling lipids identified in AH, stage/IOP-dependent alterations in glaucoma were provided by a small set of mediators, including 12,13-DiHOME, 9- and 13-HODE/KODE, arachidonic acid and lyso-PAF. These observations correlated with the expression of cytochromes P450 (CYPs) and phospholipases A2 in the ocular tissues. Interestingly, tear fluid exhibited similar lipidomic alterations in POAG. Overall, POAG may involve arachidonic acid/PAF-dependent pathways and oxidative stress as evidenced from an increase in its markers, KODEs and 12,13-DiHOME. The latter is a product of CYPs, one of which, CYP1B1, is known as POAG and primary congenital glaucoma-associated gene. These data provide novel targets for glaucoma treatment. Oxylipin content of tear fluid may have diagnostic value in POAG.

Авторы
Azbukina N.V.1 , Chistyakov D.V. 2 , Goriainov S.V. 3 , Kotelin V.I.4 , Fedoseeva E.V.4 , Petrov S.Y.4 , Sergeeva M.G. 2 , Iomdina E.N.4 , Zernii E.Y.2
Журнал
Издательство
MDPI
Номер выпуска
7
Язык
Английский
Статус
Опубликовано
Номер
658
Том
10
Год
2021
Организации
  • 1 Moscow Lomonosov State Univ, Fac Bioengn & Bioinformat, Moscow 119234, Russia
  • 2 Lomonosov Moscow State Univ, Belozersky Inst Physiochem Biol, Moscow 119992, Russia
  • 3 Peoples Friendship Univ Russia, SREC PFUR, Moscow 117198, Russia
  • 4 Helmholtz Natl Med Res Ctr Eye Dis, Moscow 105062, Russia
Ключевые слова
primary open-angle glaucoma; aqueous humor; tear fluid; lipidomics; signaling lipids; polyunsaturated fatty acids; oxylipins; phospholipid derivatives
Дата создания
16.12.2021
Дата изменения
01.08.2022
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/77416/
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