Alzheimer's soluble amyloid beta protein is secreted by HEPG2 cells as an apolipoprotein

Recently we reported that the soluble form of amyloid beta protein (sAβ) in normal human plasma and cerebrospinal fluid is associated with lipoprotein (LP) particles. In this paper we tested the sAβ secretion by cells in association with LP in the model of the human hepatoma HepG2 cell line. These cells secreted sAβ to the culture media and expressed intracellular sAβ immunoreactivity. Soluble Aβ in the cell supernatant was detected in 200-300 kDa LP complexes in association with apoA-I, apoJ, transthyrethin and phospholipids, triglycerides and free and esterified cholesterol. This was assessed by size exclusion HPLC, immunoprecipitation with corresponding antibodies and by analysis of sAβ associated metabolically-labeled lipids, respectively. Our results suggest that sAβ to LP associatio represents a unique mechanism, governing the normal biology of sAβ.

Авторы
Редакторы
-
Издательство
Wiley-Blackwell Publishing Ltd
Номер выпуска
5
Язык
Английский
Страницы
265-271
Статус
Опубликовано
Подразделение
-
Номер
-
Том
21
Год
1997
Организации
  • 1 Department of Biochemistry, Russian Academy of Medical Sciences, Russ. People's Friendship Univ. S., Miklucho-Maklay Street, 8, Moscow 117198, Russian Federation
Ключевые слова
Alzheimer's disease; Apolipoprotein; Down's syndrome; Hepatoma; Protein synthesis; Soluble amyloid beta protein
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/758/