Contradictory effect of recombinant interferon α2b on the non-transformed and transformed phenotypes of functionally signifi cant subpopulations of neutrophilic granulocytes in vitro [Heoahoihahoe bjiiiahhc peKoivi6iiHaHTHoro HHTep4)epoHa a2b Ha HeTpaHCf^opMiipoBamibiH II TpaHC^OpMIipOBaHHbUI (j)eHOTIin (|)yHKUIIOHaJIbHO 3HainiMbix cySnonyjiHunii HeiiTpo^HJibHbix rpaHyjiomiTOB b )Kcnepii\ieHTe in vitro]

Introduction. Modern studies demonstrate the multifunctionality of neutrophil granulocytes (NG) and indicate the presence of various NG subpopulations. Each of the NG subpopulations has its own phenotypic and functional features. NG subpopulations reveal plasticity and can change their phenotype under Influences of the microenvironment, exerting both positive and negative (suppressing, damaging) effects on the course of the infectious and infl ammatory process. The aim of the study was to determine the possibility of experimental reprogramming of the transformed phenotype of functionally signifi cant NG subpopulations in children with acute small purulent infection (SPI) under the Influence of the substance of recombinant interferon α2b (rIFNα2b) in the in vitro system. Material and methods. Samples of peripheral blood (PB) of 12 children of both sexes aged 2-4 years with acute small purulent infection (MGI) (the study group) and 7 conditionally healthy children, comparable in gender and age (the group of comparison), were studied. The number of NG subpopulations ?D64-CD16+CD32+CD11b+, ?D64+CD16+CD32+CD11b+, CD62L+?D63-, CD62L+?D63+ was determined by fl ow cytometry, with refi nement of their phenotype by the expression density of each studied membrane molecule according to MFI. Phagocytic and microbicidal functions of NG were studied in children of the both groups. PB samples from children of the study group and the group of comparison were incubated in the in vitro system for 1 h at 37 °? with a rIFNα2b at a fi nal concentration of 10-6 g/l, after which the features of transformation of the phenotype of NG subpopulations in both groups of children were monitored. Results. Variants of negative transformation of phenotypes of functionally signifi cant NG subpopulations were found: An increase in the number of activated CD62LdimCD63mid-NG, ?D64dimCD16midCD32dimCD11bbright-NG against the background of a decrease in the level of the major subpopulation ?D64-CD16midCD32dimCD11bbright and violations of phagocytic and microbicidal functions of NG. A subpopulation of ?D64dimCD16midCD32dimCD11bbright-NG with inadequate pro-i nfl ammatory properties was identifi ed. It was shown the possibilityof phenotype reprogramming of various NG subpopulations in study group with SPI under the Influence of rIFNα2b, which contributed to the positive regulation of the functional cell activity. Conclusion. Our study demonstrated negative transformation of the phenotype of functionally signifi cant NG subpopulations in children with SPI. The immunomodulatory effects of rIFNα2b were shown on the negatively transformed NG phenotype in study group with the local purulent process. Those effects were manifested in the switch of NG phenotype from the pro-infl ammatory to the anti-infl ammatory one. © 2020 Meditsina Publishers. All rights reserved.

Nesterova I.V. 1, 2, 4 , Chudilova Ga N.I.V. , Rusinova Tv K.S.V. , Tarakanov Va P.V.N. , Malinovskaya Vv B.N.K.
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  • 1 Kuban State Medical University, Ministry of Healthcare of Russian Federation, Krasnodar, 350063, Russian Federation
  • 2 Medical Institute, Peoples' Friendship University of Russia, Rudn University, Moscow, 117198, Russian Federation
  • 3 National Research Center of Epidemiology and Microbiology Named after N.F. Gamaleya, Ministry of Healthcare of the Russian Federation, Moscow, 123098, Russian Federation
  • 4 Department of Allergology and Immunology, Peoples' Friendship University of Russia, Rudn University, Moscow, Russian Federation
Ключевые слова
Neutrophilic granulocytes; Phenotype; Purulent infection; Subset
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