A powerful lamp weapon against the threat of the quarantine plant pathogen curtobacterium flaccumfaciens pv. Flaccumfaciens

Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is a Gram-positive phytopathogenic bacterium attacking leguminous crops and causing systemic diseases such as the bacterial wilt of beans and bacterial spot of soybeans. Since the early 20th century, Cff is reported to be present in North America, where it still causes high economic losses. Currently, Cff is an emerging plant pathogen, rapidly spreading worldwide and occurring in many bean-producing countries. Infected seeds are the main dissemination pathway for Cff, both over short and long distances. Cff remains viable in the seeds for long times, even in field conditions. According to the most recent EU legislation, Cff is included among the quarantine pests not known to occur in the Union territory, and for which the phytosanitary inspection consists mainly of the visual examination of imported bean seeds. The seedborne nature of Cff combined with the globalization of trades urgently call for the implementation of a highly specific diagnostic test for Cff, to be routinely and easily used at the official ports of entry and into the fields. This paper reports the development of a LAMP (Loop-Mediated Isothermal Amplification) specific for Cff, that allows the detection of Cff in infected seeds, both by fluorescence and visual monitoring, after 30 min of reaction and with a detection limit at around 4 fg/µL of pure Cff genomic DNA. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Авторы
Tegli S.1 , Biancalani C.1 , Ignatov A.N. 2 , Osdaghi E.3
Журнал
Издательство
MDPI AG
Номер выпуска
11
Язык
Английский
Страницы
1-13
Статус
Опубликовано
Номер
1705
Том
8
Год
2020
Организации
  • 1 Laboratorio di Patologia Vegetale Molecolare, Dipartimento di Scienze e Tecnologie Agrarie, Alimentari Ambientali e Forestali (DAGRI), Università degli Studi di Firenze, Via della Lastruccia 10, Sesto Fiorentino (Firenze), 50019, Italy
  • 2 Agrarian and Technological Institute (ATI), Peoples’ Friendship University of Russia, Miklukho-Maklaya str.8, Moscow, 117198, Russian Federation
  • 3 Department of Plant Protection, University of Tehran, Karaj, 31587-77871, Iran
Ключевые слова
Bacterial wilt of bean; Curtobacterium flaccumfaciens pv. flaccumfaciens; LAMP; Loop-Mediated Isothermal Amplification; Molecular diagnostics; Quarantine plant pathogen; Tan spot of soybean
Дата создания
20.04.2021
Дата изменения
20.04.2021
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/72601/
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