In vitro and in vivo studies of biodegradability and biocompatibility of poly(εcl)-b-poly(etoep)-based films

The control of surface bioadhesive properties of the subcutaneous implants is essential for the development of biosensors and controlled drug release devices. Poly(alkyl ethylene phosphate)-based (co)polymers are structurally versatile, biocompatible and biodegradable, and may be regarded as an alternative to poly(ethylene glycol) (PEG) copolymers in the creation of antiadhesive materials. The present work reports the synthesis of block copolymers of ε-caprolactone (εCL) and 2-ethoxy-1,3,2-dioxaphospholane-2-oxide (ethyl ethylene phosphate, EtOEP) with different content of EtOEP fragments, preparation of polymer films, and the results of the study of the impact of EtOEP/εCL ratio on the hydrophilicity (contact angle of wetting), hydrolytic stability, cytotoxicity, protein and cell adhesion, and cell proliferation using umbilical cord multipotent stem cells. It was found that the increase of EtOEP/εCL ratio results in increase of hydrophilicity of the polymer films with lowering of the protein and cell adhesion. MTT cytotoxicity test showed no significant deviations in toxicity of poly(εCL) and poly(εCL)-b-poly(EtOEP)-based films. The influence of the length of poly(EtOEP)chain in block-copolymers on fibrotic reactions was analyzed using subcutaneous implantation experiments (Wistar line rats), the increase of the width of the fibrous capsule correlated with higher EtOEP/εCL ratio. However, the copolymer-based film with highest content of polyphosphate had been subjected to faster degradation with a formation of developed contact surface of poly(εCL). The rate of the degradation of polyphosphate in vivo was significantly higher than the rate of the degradation of polyphosphate in vitro, which only confirms an objective value of in vivo experiments in the development of polymer materials for biomedical applications. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Nifant’ev I.1, 2, 3 , Shlyakhtin A.1 , Komarov P.2 , Tavtorkin A.2 , Kananykhina E.4 , Elchaninov A. 5 , Vishnyakova P. 5 , Fatkhudinov T. 4, 6 , Ivchenko P.1, 2
Номер выпуска
  • 1 Chemistry Department, M.V. Lomonosov Moscow State University, 1-3 Leninskie Gory, Moscow, 119991, Russian Federation
  • 2 A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., Moscow, 119991, Russian Federation
  • 3 Faculty of Chemistry, National Research University Higher School of Economics, 20 Miasnitskaya Str., Moscow, 101000, Russian Federation
  • 4 Research Institute of Human Morphology, 3 Tsyurupy St., Moscow, 117418, Russian Federation
  • 5 National Medical Research Center for Obstetrics Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 4 Oparina Str., Moscow, 117997, Russian Federation
  • 6 Faculty of Science, Peoples’ Friendship University of Russia, Miklukho-Maklaya 6 Str., Moscow, 117198, Russian Federation
Ключевые слова
Cytotoxicity; Immunohistochemical test; Polycaprolactone; Polyesters; Polyphosphoesters; Protein adsorption; Ring-opening polymerization
Дата создания
Дата изменения
Постоянная ссылка

Другие записи