Ultrasound-assisted esterification of valeric acid to alkyl valerates promoted by biosilicified lipases

A novel, environmentally friendly, and sustainable ultrasound-assisted methodology in the valorization of valeric acid to alkyl valerate using a biosilicified lipase from Candida antarctica is reported. This one-pot room temperature methodology of enzyme biosilicification leads to biosilicified lipases with improved activity and reaction efficiency as compared to free enzymes. Yields in the ultrasound-promoted esterification of valeric acid was ca. 90% in 2 h with 15% m/v of biosilicified lipase (Bio-lipase; 616 U/g biocatalyst enzymatic activity) and a molar ratio 1:2 (valeric acid:ethanol), slightly superior to that observed by the free enzyme (75% conversion, 583U/g biocatalyst enzymatic activity). The reuse of enzymes in these conditions was tested and the results show a relatively good reusability of these biosilicified enzymes under the investigated conditions, particularly preserving fairly stable specific activities (616 vs. 430 U/g biocatalyst after four reuses). © 2018 Cebrián-García, Balu and Luque.

Авторы
Cebrián-García S.1 , Balu A.M.1 , Luque R. 1, 2
Журнал
Издательство
Frontiers Media S. A
Номер выпуска
JUN
Язык
Английский
Статус
Опубликовано
Номер
197
Том
6
Год
2018
Организации
  • 1 Departamento de Quimica Organica, Universidad de Cordoba, Cordoba, Spain
  • 2 Scientific Center for Molecular Design and Synthesis of Innovative Compounds for the Medical Industry, Peoples Friendship University of Russia (RUDN), Moscow, Russian Federation
Ключевые слова
Biocatalysis; Biosilicification; Esterification; Lipases; Ultrasounds
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/7022/