Phosphazide metabolism studies on the rats inverted intestine

In this work the intestinal metabolism of phospazide was demonstrated using rats inverted intestine model with phosphate buffer pH 6.8 at 37 °Ñ. Freshly dissected small intestine of a laboratory rat was used. Metabolism was studied at two levels of phosphazide concentrations of 20 ìg/ml and 2 ìg/ml for two replicates. Sample time points 0, 2, 5, 17 hours. Samples were prepared with protein precipitation using a 1 % solution of ammonia in methanol and centrifugation, the analysis of the samples was carried out using HPLC-UV. According the results of this study, it can be concluded that there is a mechanism that transforms phosphazide to zidovudine on the surface of the small intestinal epithelium. Presumably this mechanism can play an important role in the pharmacokinetics of phosphazide. © 2019, Folium Ltd. All rights reserved.

Авторы
Grebenkin D.Y. 1, 2 , Stanishevskiy Y.M. 1 , Shohin I.E. 3 , Brovchenko B.V.4 , Smirnov A.A.4 , Malashenko E.A.5
Издательство
Общество с ограниченной ответственностью Фолиум
Номер выпуска
2
Язык
Русский
Страницы
3-7
Статус
Опубликовано
Том
11
Год
2019
Организации
  • 1 Institute of Biochemical Technology and Nanotechnology, Peoples’ Friendship University of Russia, 10-2, Miklukho-Maklaya St., Moscow, 117198, Russian Federation
  • 2 ExacteLabs Company, 20, Nauchnyi proezd, Moscow, 117246, Russian Federation
  • 3 Center of Pharmaceutical Analytics Ltd, 20, Nauchniy Proezd, Moscow, 117246, Russian Federation
  • 4 LLC «AZT PHARMA K. B.», 15A, 3-rd, Cherepkovskaya str., Moscow, 121552, Russian Federation
  • 5 I. M. Sechenov First Moscow State Medical Univercity (Sechenov University), 2, Bolshaya Pirogovskaya str., Moscow, 119435, Russian Federation
Ключевые слова
Bioavailability; Intestine; Metabolism; Permeability; Phosphazide; Zidovudine
Дата создания
02.11.2020
Дата изменения
02.11.2020
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/65820/
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Вопросы истории. Общество с ограниченной ответственностью "Журнал "Вопросы истории". Том 2019. 2019. С. 49-53