It is known that one of the main pathogenetic mechanisms of male infertility is increasing the level of reactive oxygen species (ROS) in the ejaculate. Thereby identify of pathogenetic factors of oxidative stress in pathospermia is very actually. A retrospective analysis of 230 men with poor semen quality (according to criteria of semen, WHO 2010) and increased ROS in the native ejaculate was conducted. The highest amount of ROS in infertile men was in combination with varicocele and chronic prostatitis category 2. ROS level was significantly higher (p<0.05) in men using a mobile phone compared to patients with bad habits (alcohol use). Thus identify the pathogenesis of ROS formation in the ejaculate to choose the right pathogenetic treatment of male infertility, as well as to use preventive measures to combat pathospermia. © Group of authors, 2019.