Comparative Analysis of the Different Dyes' Potential to Assess Human Normal and Cancer Cell Viability in Vitro under Different D / H Ratios in a Culture Medium

In this study, using new approach (laser diffraction + biological dyes), we have demonstrated the decrease of cells viability in vitro in the deuterated growth medium, whereas in the deuterium-depleted medium, there was an increase of cell viability. We have also found that not all dyes are equally sensitive to the D/H ratios in the culture medium (system) as well as to the different cell types (cancer vs normal cells). © 2020 I. A. Zlatskiy et al.

Zlatskiy I.A. 1, 2 , Zlatska A.V.2, 3 , Antipova N.V. 1, 4 , Dolenko S.A.5 , Gordiienko I.M.3, 6 , Gubar O.S.3 , Vasyliev R.G.2, 3 , Zubov D.A.2 , Novikova S.N.2 , Syroeshkin A.V. 1
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  • 1 Peoples Friendship University of Russia, RUDN University, 6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation
  • 2 State Institute of Genetic and Regenerative Medicine NAMS of Ukraine, 67 Vyshgorodska Str., Kyiv, 04114, Ukraine
  • 3 Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya, Kiev, Ukraine
  • 4 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation
  • 5 Institute of Colloid and Water Chemistry, National Academy of Sciences of Ukraine, Kyiv, 03680, Ukraine
  • 6 RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Ключевые слова
deuterium; pantoprazole; coloring agent; deuterium; A-549 cell line; Article; cell differentiation; cell viability; chemical parameters; controlled study; electric conductivity; human; human cell; human experiment; in vitro study; laser diffraction; normal human; oxidation reduction potential; particle size; physical parameters; analysis; cell culture; cell culture technique; cell line; cell survival; chemical phenomena; chemical structure; chemistry; culture medium; drug effect; laser; tumor cell line; Cell Culture Techniques; Cell Line; Cell Line, Tumor; Cell Survival; Cells, Cultured; Chemical Phenomena; Coloring Agents; Culture Media; Deuterium; Humans; Lasers; Molecular Structure; Particle Size
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