Effect of nanocarriers of different nature on the immunological characteristics of immobilized monoclonal autoantibodies to thyroglobulin

The immunological properties of monoclonal autoantibodies to thyroid thyroglobulin in the construction of carrier-bioligand conjugates have been studied. Silver and gold nanoparticles 40-50 nm in size of and polymer microspheres (706 nm) were obtained. Autoantibodies were immobilized on the surface of the synthesized nanoparticles and the effect of nanocarriers on the immunological properties of immobilized monoclonal autoantibodies to thyroglobulin was studied by ELISA. It was found that the use of silver and gold nanoparticles allows you to adjust the sensitivity and specificity of the enzyme-linked immunosorbent assay in order to increase or decrease the detection limit of antigens or antibodies for early laboratory diagnostics of diseases and the creation of new-generation diagnostic test systems. © 2020.

Редакторы
-
Издательство
State Research Institute for Genetics and Selection of Industrial Microorganisms
Номер выпуска
4
Язык
Русский
Страницы
106-111
Статус
Опубликовано
Подразделение
-
Номер
-
Том
36
Год
2020
Организации
  • 1 Peoples Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
  • 2 Research Institute of Vaccines and Serums named after I.I. Mechnikov, Moscow, 105064, Russian Federation
Ключевые слова
Gold; Human thyroid gland thyroglobulin; Immunodiagnostics; Nanocarriers; Nanoparticles; Polystyrene; Silver
Дата создания
02.11.2020
Дата изменения
02.11.2020
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/65199/