Post-chromatographic fixed-charge derivatization for the analysis of hydroxyl-containing compounds by a combination of thin-layer chromatography and matrix-assisted laser desorption/ionization mass spectrometry

A simple and convenient on-spot derivatization has been suggested for the modification of hydroxyl-containing compounds for their analysis by thin layer chromatography/matrix-assisted laser desorption ionization mass spectrometry (TLC/MALDI). The proposed approach was based on post-chromatographic acylation of separated analytes by 3-bromopropionyl chloride with simultaneous quaternization of pyridine. In contrast to the initial alcohols not ionizable in TLC/MALDI conditions, the derivatives, containing permanent positive charge, revealintense peaks of their cationic moieties in MALDI mass spectra recorded directly from TLC plates. The method was tested on a series of mammalian and plant sterols, phenols and terpene alcohols. © 2018 Elsevier B.V.

Авторы
Esparza C. 2 , Borisov R.S. 2, 1 , Polovkov N.Y.1 , Zaikin V.G. 1
Редакторы
-
Издательство
-
Номер выпуска
-
Язык
Английский
Страницы
97-103
Статус
Опубликовано
Подразделение
-
Номер
-
Том
1560
Год
2018
Организации
  • 1 A.V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninskii pr. 29, Moscow, 119991, Russian Federation
  • 2 Peoples’ Friendship University of Russia, Mikluho-maklaya st.6, Moscow, 117198, Russian Federation
Ключевые слова
Alcohols; Derivatization; MALDI; Mass spectrometry; Quaternization.; TLC
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/6506/