The biosynthesis of L-lysine-α-oxidase from Trichoderma harzianum Rifai F-180, an antitumor enzyme producer, was carried out on a wheat bran medium. Hydrophilic bases of lightly crosslinked acrylic polymers and methylcellulose derivatives were selected, and model samples were prepared with a 1% concentration of the culture liquid of the fungus. The wound healing activity of the obtained gel samples was investigated on an experimental model of guinea pig wounds. The results of experimental studies showed a high wound healing activity of the gel with a 1% concentration of the culture liquid of the fungus and can serve as a basis for further preclinical trials of this dosage form of wound healing action. © 2020 EManuscript Technologies. All rights reserved.