Comparative lipidomic analysis of inflammatory mediators in the aqueous humor and tear fluid of humans and rabbits

Introduction: Ocular inflammation is a key pathogenic factor in most blindness-causing visual disorders. It can manifest in the aqueous humor (AH) and tear fluid (TF) as alterations in polyunsaturated fatty acids (PUFAs) and their metabolites, oxylipins, lipid mediators, which are biosynthesized via enzymatic pathways involving lipoxygenase, cyclooxygenase or cytochrome P450 monooxygenase and specifically regulate inflammation and resolution pathways. Objectives: This study aimed to establish the baseline patterns of PUFAs and oxylipins in AH and TF by their comprehensive lipidomic identification and profiling in humans in the absence of ocular inflammation and comparatively analyze these compounds in the eye liquids of rabbits, the species often employed in investigative ophthalmology. Methods: Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used for qualitative and quantitative characterization of lipid compounds in the analyzed samples. Results: A total of 28 lipid compounds were identified, including phospholipid derivatives and PUFAs, as well as 22 oxylipins. Whereas the PUFAs included arachidonic, docosahexaenoic and eicosapentaenoic acids, the oxylipins were derived mainly from arachidonic, linoleic and α-linolenic acids. Remarkably, although the concentration of oxylipins in AH was lower compared to TF, these liquids showed pronounced similarity in their lipid profiles, which additionally exhibited noticeable interspecies concordance. Conclusion: The revealed correlations confirm the feasibility of rabbit models for investigating pathogenesis and trialing therapies of human eye disorders. The identified metabolite patterns suggest enzymatic mechanisms of oxylipin generation in AH and TF and might be used as a reference in ocular inflammation studies. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.

Авторы
Chistyakov D.V. 1 , Azbukina N.V.1, 2 , Astakhova A.A.1 , Goriainov S.V. 3 , Chistyakov V.V. 3 , Tiulina V.V.1 , Baksheeva V.E.1 , Kotelin V.I.4 , Fedoseeva E.V.4 , Zamyatnin A.A.Jr. , Philippov P.P.1 , Kiseleva O.A.4 , Bessmertny A.M.4 , Senin I.I.1 , Iomdina E.N.4 , Sergeeva M.G. 1 , Zernii E.Y.1, 5
Журнал
Издательство
Springer
Номер выпуска
2
Язык
Английский
Статус
Опубликовано
Номер
27
Том
16
Год
2020
Организации
  • 1 Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, 119992, Russian Federation
  • 2 Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119234, Russian Federation
  • 3 Peoples’ Friendship University of Russia, Moscow, 117198, Russian Federation
  • 4 Helmholtz National Medical Research Center of Eye Diseases, Moscow, 105062, Russian Federation
  • 5 Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow, 119991, Russian Federation
Ключевые слова
Aqueous humor; COX; CYP450; LOX; Oxylipins; PUFAs; Tear fluid; UPLC-MS/MS
Дата создания
02.11.2020
Дата изменения
02.11.2020
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/64975/
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