The development of an HPLC method for quantitative determination of meropenem in human blood plasma for therapeutic drug monitoring in clinics is reported. Spectrophotometric detection was made at 298 nm. The chromatography time was 12 min. The method was validated for the basic parameters and was linear over a wide concentration range (from 0.2 to 150 ìg/mL). The lower limit of quantitation was 0.2 ìg/mL, which provided the required method sensitivity. The correlation coefficient of the calibration curve was 0.9998. The developed method was shown to be applicable for real-time monitoring of the meropenem content in patient blood plasma, which allowed the dose to be personalized and the efficacy and rationality of the antibacterial therapy to be increased. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.