Development and Validation of a Quantitative Determination Method for Meropenem in Blood Plasma for Therapeutic Drug Monitoring

The development of an HPLC method for quantitative determination of meropenem in human blood plasma for therapeutic drug monitoring in clinics is reported. Spectrophotometric detection was made at 298 nm. The chromatography time was 12 min. The method was validated for the basic parameters and was linear over a wide concentration range (from 0.2 to 150 ìg/mL). The lower limit of quantitation was 0.2 ìg/mL, which provided the required method sensitivity. The correlation coefficient of the calibration curve was 0.9998. The developed method was shown to be applicable for real-time monitoring of the meropenem content in patient blood plasma, which allowed the dose to be personalized and the efficacy and rationality of the antibacterial therapy to be increased. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.

Авторы
Kazanova A.M. 1, 3 , Stepanova E.S. 2 , Makarenkova L.M. 2 , Chistyakov V.V. 2 , Zyryanov S.K. 3, 4 , Senchenko S.P.1
Номер выпуска
4
Язык
Английский
Страницы
414-418
Статус
Опубликовано
Том
54
Год
2020
Организации
  • 1 Scientific Center for Expert Evaluation of Medicinal Products, Ministry of Health of the Russian Federation, 8/2 Petrovskii Blvd., Moscow, 127051, Russian Federation
  • 2 Common Use Center (Research and Educational Center), People’s Friendship University of Russia, 8 Miklukho-Maklaya St., Moscow, 117198, Russian Federation
  • 3 People’s Friendship University of Russia, 10/2 Miklukho-Maklaya St., Moscow, 117198, Russian Federation
  • 4 Municipal Clinical Hospital No. 24, Moscow City Health Department, 10 Pistsovaya St., Moscow, 127015, Russian Federation
Ключевые слова
blood plasma; carbapenems; HPLC-UV method; meropenem
Дата создания
02.11.2020
Дата изменения
02.11.2020
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/64578/
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