The mass-spectrometric IRMS/SIRA (SIRA – Stable Isotope Ratio Analysis) technique was applied to study the biological and technogenic fractionation of stable isotopes of light elements - carbon ( 13 C/ 12 C) in components of C3-plants by the example of grape (Vitisvinifera L.) grown in climatic conditions of modern natural ecosystems including urban ecosystems of agrarian regions. The carbon isotopes ratios 13 C/ 12 C in carbohydrates and ethanol in authentic samples of grapes and wines were studied by the FC-IRMS/SIRA method. The main goal of the conducted study was the determination of the correlation between specific climatic conditions of the C3-plants growing ecosystems and stable isotopes ratios in components of grapes and wines. The results of the study demonstrate the influence of climatic characteristics of natural ecosystems and technogenic factors (e.g. processing technologies) on the quantitative levels of the composition of stable isotopes of light elements. The publication has been prepared with the support of the «RUDN University Program 5-100». © 2020, Springer Nature Switzerland AG.