Inhibiting bacterial cooperation is an evolutionarily robust anti-biofilm strategy

Bacteria commonly form dense biofilms encased in extracellular polymeric substances (EPS). Biofilms are often extremely tolerant to antimicrobials but their reliance on shared EPS may also be a weakness as social evolution theory predicts that inhibiting shared traits can select against resistance. Here we show that EPS of Salmonella biofilms is a cooperative trait whose benefit is shared among cells, and that EPS inhibition reduces both cell attachment and antimicrobial tolerance. We then compare an EPS inhibitor to conventional antimicrobials in an evolutionary experiment. While resistance against conventional antimicrobials rapidly evolves, we see no evolution of resistance to EPS inhibition. We further show that a resistant strain is outcompeted by a susceptible strain under EPS inhibitor treatment, explaining why resistance does not evolve. Our work suggests that targeting cooperative traits is a viable solution to the problem of antimicrobial resistance. © 2020, The Author(s).

Авторы
Dieltjens L.1 , Appermans K.1 , Lissens M.1 , Lories B.1 , Kim W.2, 3 , Van Der Eycken E.V. , Foster K.R.2 , Steenackers H.P.1, 2
Журнал
Издательство
Nature Publishing Group
Номер выпуска
1
Язык
Английский
Статус
Опубликовано
Номер
107
Том
11
Год
2020
Организации
  • 1 Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Leuven, Belgium
  • 2 Department of Zoology and Department of Biochemistry, University of Oxford, Oxford, United Kingdom
  • 3 Department of Biological Sciences, Duquesne University, Pittsburgh, United States
  • 4 Department of Chemistry, Laboratory for Organic & Microwave-Assisted Chemistry (LOMAC), KU Leuven, Leuven, Belgium
  • 5 Peoples’ Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya street, Moscow, Russian Federation
Ключевые слова
2 cyclopentenyl 5 (4 chlorophenyl) 2 aminoimidazole; bacterial protein; ciprofloxacin; crystal violet; curlin; hydrogen peroxide; imidazole derivative; spectinomycin; unclassified drug; antimicrobial activity; bacterium; biofilm; cell; experimental study; inhibition; inhibitor; polymer; antibiofilm activity; antibiotic resistance; antibiotic sensitivity; Article; bacterial colonization; bacterial growth; bacterium adherence; bacterium culture; biofilm; biofilm matrix; biomass; biomass production; cell adhesion; colony formation; colony forming unit; confocal laser scanning microscopy; controlled study; cooperation; gene deletion; gene expression; growth rate; nonhuman; operon; Salmonella; Salmonella enterica serovar Typhimurium; social evolution; transcription initiation; Bacteria (microorganisms); Salmonella
Дата создания
10.02.2020
Дата изменения
10.02.2020
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/56489/