Aim: The biosynthesis of L-lysine-α-oxidase enzyme by Trichoderma fungus has been studied at various cultivation methods and on the media with various carbon sources. In the work presented, various variants of media and the possibility of wheat bran and hemicellulose substrate replacement by other carbon sources in connection with an enzyme development were studied, as well as the substrate specificity of Trichoderma during the use of this substrate depending on the culture and acidity medium age. Materials and Methods: The vegetative mycelium of mushrooms, which was grown in 8-10 days at t - 28°C on the Chapek medium, served as a seed material. The study was carried out in a deep-seated manner using the laboratory shaker No. 357 ("Poland") and a surface method of cultivation. Results: The enzyme was synthesized actively only on the medium with wheat bran. Trichoderma strains, when they were grown on the medium with a hemicellulose substrate, exhibited a different spectrum of L-amino acid oxidase activities. With the age of the Trichoderma culture, the spectrum of aminoxidase activities expanded, but it remained individual for each strain and could serve as a marker for its characteristics. Conclusion: As can be seen from the presented results, a specific spectrum of amino acid degradation manifests itself for each strain of various representatives of Trichoderma - the producers of L-lysine-a-oxidase, which can serve as a biochemical characteristic of the strain. © 2018 Association for Computing Machinery. All rights reserved.