Study of baicalin hydrolysis kinetics in the process of its extraction from Scutellaria baicalensis Georgi roots

The aim of this study was to investigate the kinetics of baicalin hydrolysis in the process of its extraction from Scutellaria baicalensis Georgi roots. Materials and methods. For the studies, Scutellaria baicalensis Georgi roots with a particle range of 0.1.0.5 mm were used. The method of extraction was a simple maceration during a specified period of time, the ratio of plant raw material : extractant was 1:10 w/v at the temperature of 24±1°C. Baicalin and baicalein contents were analyzed by reverse phase high performance liquid chromatography (RP HPLC) at the analytical wavelength of 275 nm. The extractant was a water solution of ethanol 26, 43, 59, 72, 81, 97±1% v/v. The time of the extraction was from 1 to 24 hours. Results. The experimental points of dependency of baicalin concentration in the extract on the time of extraction for ethanol solutions with a concentration of 43 and 72% v/v are closely approximated by a linear equation in coordinates lnC=f(t). The value of determination coefficient is more than R2>0,99. Half lifetime for baicalin has been calculated: for ethanol with the concentration of 43% v/v it is 4.3±0.7 hours, and for ethanol with the concentration of 72% v/v it is 42.3±1.8 hours. Conclusion. Baicalin hydrolysis kinetics in the process of its extraction from Scutellaria baicalensis Georgi roots with 43 and 72% v/v ethanol concentration. has been studied. It has been established that the process of baicalin hydrolysis is well described by the first order kinetic equation. The constants of baicalin hydrolysis during its extraction from Scutelaria baicalensis roots with ethanol having different concentrations have been calculated. Recommendations on technology optimization for baicalin or baicalein extraction from Scutellaria baicalensis Georgi roots have been given. © 2019 Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute. All rights reserved.

Авторы
Boyko N.N.1 , Pisarev D.I.1 , Zhilyakova E.T.1 , Maljutina A.Y.1 , Novikov O.O. 2 , Bocharnikova M.A.1
Издательство
Пятигорский медико-фармацевтический институт - филиал федерального бюджетного образовательного учреждения высшего образования "Волгоградский государственный медицинский университет" Министерства здравоохранения Российской Федерации
Номер выпуска
3
Язык
Английский
Страницы
129-137
Статус
Опубликовано
Том
7
Год
2019
Организации
  • 1 Belgorod National Research University, 85, Pobeda St., Belgorod, 308015, Russian Federation
  • 2 Peoples' Friendship University of Russia, 8/2, Miklukho-Maklay St., Moscow, 117198, Russian Federation
Ключевые слова
Baicalein; Baicalin; First order reaction; Half lifetime; Hydrolysis; Scutellaria baicalensis Georgi roots
Дата создания
24.12.2019
Дата изменения
19.03.2021
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/55369/
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Другие записи

Umirova A.A., Adzhienko V.L., Kabakova T.I., Korzhavykh E.A., Gatsan V.V.
Фармация и фармакология. Пятигорский медико-фармацевтический институт - филиал федерального бюджетного образовательного учреждения высшего образования "Волгоградский государственный медицинский университет" Министерства здравоохранения Российской Федерации. Том 7. 2019. С. 158-169