Synthesis, biological activity and molecular modeling of a new series of condensed 1,2,4-triazoles

A ring transformation of 6-methyl-7H[1,2,4]triazolo [4,3-b][1,2,4] triazepine-8(9H)-ones (thiones) in the presence of acetic anhydride give rise to a new series of 17 condensed 1,2,4-triazole derivatives (1–17). Plausible mechanisms are proposed and show the formation of a beta fused β-lactam moiety. The compounds were tested for their (i) inhibitory potential on digestive enzymes (α-amylase and α-glucosidase), and (ii) antioxidant activity using radical scavenging (DPPH and ABTS radicals) and ferric reducing power assays. The compounds showed interesting and promising antidiabetic activities compared to the reference drug Acarbose. Molecular docking study has been carried out to determine the binding mode interactions between these derivatives and the targeted enzymes. The results showed the strength of intermolecular hydrogen bonding in ligand-receptor complexes as an important descriptor in rationalizing the observed inhibition results. Moreover, molecular dynamics simulations are also performed for the best protein-ligand complex to understand the stability of small molecule in a protein environment. To shed light on the antioxidant activity of the synthesized compounds and the mechanism involved in DPPH free radical, DFT calculations were performed at the B3P86/6-311++G(d,p) level using the polarizable continuum model. The effect of aprotic solvent on bond dissociation enthalpies (BDEs) is investigated by calculating and comparing BDEs of 1 in methanol and dimethylsulfoxide as solvents using PCM. The obtained results show that the mechanism of action depends on the basic skeleton and the presence of substituted functional groups in these derivatives. BDEs are found to be slightly influenced by the aprotic solvent of less than 0.01 kcal/mol compared with those obtained in methanol. © 2019 Elsevier Inc.

El Bakri Y. , Marmouzi I.3 , El Jemli M. , Anouar E.H.4 , Karthikeyan S. 2 , Harmaoui A.1 , Faouzi M.E.A.3 , Mague J.T.5 , Essassi E.M.1
Academic Press Inc.
  • 1 Laboratoire de Chimie Organique Hétérocyclique, Centre de Recherche des Sciences des Médicaments, Pôle de Compétences Pharmacochimie, URAC 21, Faculté des Sciences, Université Mohammed V Rabat, Avenue Ibn Battouta, BP 10014, Rabat, Morocco
  • 2 Organic Chemistry Department, Science Faculty, RUDN University Miklukho-Maklaya st. 6, Moscow, 117198, Russian Federation
  • 3 University Mohammed V in Rabat, Faculté de Médicine et de Pharmacie, Laboratoire de Pharmacologie et Toxicologie, équipe de Pharmacocinétique, BP 6203, Rabat Instituts, Rabat, Morocco
  • 4 Department of Chemistry, College of Sciences and Humanities, Prince Sattam bin Abdulaziz University, P.O. Box 83, Al Kharj, 11942, Saudi Arabia
  • 5 Department of Chemistry, Tulane University, New Orleans, LA 70118, United States
Ключевые слова
1,2,4-Triazoles; Antidiabetic; Antioxidant activity; DFT calculations; Docking study; Molecular dynamics; Pyrazolotriazole
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