HPLC-UV Method for Determing Flavonoids in Hawthorn Flowers and Leaves

An HPLC technique with UV spectrophotometric detection was developed for qualitative and quantitative determination of vitexin, rutin, hyperoside, and quercetin in Crataegus sanguinea hawthorn herbs (flowers and leaves). The separation used a Luna C18(2) column (4.6 × 150 mm, 5 μm) and a stepwise gradient. Calibration curves for the four flavonoids exhibited good linearity (r2> 0.995) in the studied ranges. The analytical recoveries over one day/week were 95.9-104.3%/95.6 – 102.9% for vitexin; 94.0 – 102.2%/93.5 – 101.5% for rutin; 98.0 – 102.2%/97.5 – 102.9% for hyperoside; and 96.0 – 109.8%/97.2 – 111.0% for quercetin. The intra- and interday coefficients of variation (RSD, %) were <3%. The relative error (ε, %) of a single determination at confidence level 95% was <3% for rutin, hyperoside, and vitexin and <4% for quercetin. © 2017, Springer Science+Business Media, LLC.

Авторы
Sagaradze V.A.1 , Babaeva E.Y. 2 , Kalenikova E.I.1
Редакторы
-
Издательство
-
Номер выпуска
4
Язык
Английский
Страницы
277-280
Статус
Опубликовано
Подразделение
-
Номер
-
Том
51
Год
2017
Организации
  • 1 Department of Basic Medicine, M. V. Lomonosov Moscow State University, Moscow, 119991, Russian Federation
  • 2 Agro-Technological Institute, People’s Friendship University of Russia, Moscow, 117198, Russian Federation
Ключевые слова
Crataegus sanguinea; flavonoids; HPLC-UV; hyperoside; quercetin; rutin; vitexin
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/5432/