DETECTION OF THE EFFECT OF GFP ON CELLS AND THE INFLUENCE OF GENE ENGINEERING MANIPULATIONS ON CHICKEN EMBRYOS
Information about the methods of detection of the effect of GFP on cells and influence of genetic engineering on the morphometric trials of chick embryos were studied. Green fluorescent protein (GFP) is rapidly becoming one of the most frequently employed molecular reporters. Its use in monitoring gene expression and protein localization has been well documented. For all morphological parameters, the difference was statistically unreliable. On the length of the torso the difference varied from 0,3 to 5,6%, the length of the head from 0,25 to 5,7%, the length of the neck from 0,35 to 5,8%. Lower length values for all parameters were recorded in eggs obtained from a non-transgenic bird. The change in the bird's genotype did not affect the morphological indices of embryos during incubation. From 3 to 7 days of incubation, the length indicators increased by an average of 2.4 times, and the width by 8.1 times. From 7 to 10 days of incubation, the length increased by an average of 30.7%, and the width by 32,4%.