Investigation of the bischofite gel reparative effect on the linear wound model
Introduction. The currently available information on the bischofite mineral pharmacodynamics makes it possible to assume that bischofite-based gels have wound-healing properties. Materials and methods. Experiments were performed on 36 male rats. Using a blade, a linear wound of 50 mm long was modeled. Animals were divided into 4 groups: 1) Control; 2) Bishofit (500 mg of gel with bischofite on the wound area for 7 days); 3) Actovegine (500 mg of Actovegine gel on the wound area for 7 days); 4) Contractubex (500 mg of contractubex gel on the wound area for 7 days). The wound healing effect of the drugs was evaluated through studying the physicomechanical properties, assessing the morphological picture, determining the concentration of hydroxyproline (HP) and calculating the ratio of types I and III collagen. Results. The strongest healing effect on the wound defect, significantly greater than that in the control (p<0.01), was obtained in the group that received bischofite gel (13.70±0.76 N). Actovegine also demonstrated a positive reparative effect (12.60±0.63 N, p<0.05). Significantly lower (p<0.01) healing effect than in the control group was obtained in the group of animals that had received contractubex (9.65±0.59 N). In the calorimetric analysis, it was found that the highest concentration of HP was in the tissues of wound defects in animals treated with contractubex; however, there was no statistically significant difference with the control group. Significantly lower in comparison with the control (p<0.05) concentration of HP, was found in tissues of simulated wounds in animals treated with bischofite gel (79.7% of the control). When assessing the ratio of types I and III collagen in the tissues of the wound defect, when stained with picrosirius red, it was found that by the number of mature collagen fibers, the studied groups can be arranged in the following descending order: Bishofite> Actovegine> Control> Contractubex. A similar trend is demonstrated by the morphological picture of tissues in the area of the wound defect. Conclusion. The study showed that the best results were obtained with external use of bischofite gel. Actovegine has a less significant, but quite pronounced reparative, effect on this model. The least satisfactory results were obtained when applying contractubex.