Fluorescence enhancing of 5,10,15,20-tetraphenylporphyrin in complex with human serum albumin and gold nanorods

Using absorption and fluorescence spectroscopy methods we obtained the results demonstrating alterations in spectral characteristics in supramolecular system composed of gold nanorods (AuNR) (10 × 38 nm) and complexes of human serum albumin (HSA) and 5,10,15,20-tetraphenylporphyrin (TPP). TPP fluorescence (λmax = 636 and 658 nm) was found to enhance. The dependence of fluorescence enhancing in time was of nonlinear nature. Maximum TPP fluorescence enhancing value was as high as 16% and it was achieved in 7 min after mixing the components. Simultaneously with TPP fluorescence enhancing we observed a decrease in HSA own fluorescence (λmax = 340 nm) and optical density reduction in maximum of longitudinal localized plasmon band of AuNR (λmax = 752 nm). © 2014, Pleiades Publishing, Inc.