The effect of pyruvate on antibody interaction with group-specific erythrocyte antigens

Using the AB0 antibody-antigen model the influence of natural metabolite pyruvate on the antibody interaction with of erythrocyte antigens, defining their group specificity has been investigated. Before agglutination reaction erythrocytes of A (II)-AB (IV) blood groups, monoclonal anti-A and anti-B antibodies were incubated with sodium pyruvate. Visualization of agglutinates was performed by means of flow cytometry and laser scanning confocal microscopy. Computer-aided prediction of the spectrum of biological activity of pyruvate by a PASS program proposed major regulatory pathways, in which pyruvate may be involved. It has been demonstrated that pyruvate can regulate the intensity of antigen-antibody interaction. These results suggest the possibility of using small molecules, for example pyruvate, as molecular probes and prospects of the use of erythrocytes with antigenic determinants of the ABO system expressed on their membranes for studies of protein-protein interactions due to convenient visualization and possibility of quantitative evaluation of this process. © 2014 Pleiades Publishing, Ltd.

Авторы
Gylmiyarova F.N.1 , Radomskaya V.M.1 , Gusyakova O.A.1 , Ryskina E.A. 2 , Kolotyeva N.A.1 , Shahnovich E.A.1 , Nefedova N.S.1 , Sidorova I.F.1 , Baisheva G.M.1 , Pervova Yu.Y.1 , Pleten A.P.3
Номер выпуска
3
Язык
Английский
Страницы
260-266
Статус
Опубликовано
Том
8
Год
2014
Организации
  • 1 Department of Fundamental and Clinical Biochemistry with Laboratory Diagnostics, Samara State Medical University, ul. Chapaevskaya 89, Samara, 443099, Russian Federation
  • 2 Russian Peoples' Friendship University, Moscow, Russian Federation
  • 3 Evdokimov Moscow State University of Medicine and Dentistry, Moscow, Russian Federation
Ключевые слова
AB0 antigens; confocal microscopy; monoclonal antibodies; protein-protein interaction; pyruvate
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/4993/
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