Phenotypic characteristics of the monocyte subpopulations CD64+CD16-CD32+CD11b+, CD64+CD16+ CD32+CD11b+, CD64-CD16+ CD32+CD11b+ in deeply premature newborns with congenital pneumonia
Early detection of infectious-inflammatory and purulent-septic processes in deeply premature newborns is very difficult and extremely urgent in modern neonatology. The aim of our study was to identify the phenotypic characteristics of subpopulations CD64+CD16-CD32+CD11b+, CD64+CD16+CD32+CD11b+, CD64-CD16+CD32+CD11b+ monocytes (Mon) with definition of their diagnostic value in deeply premature newborns with the congenital pneumonia varying severity and the respiratory distress syndrome. There was investigated a momentary expression of molecules DD64, CD32, DD16, CD11b on the surface membrane of Mon by the flow cytometry. The testing of the level of their density was done, using detection of the mean intensity of the fluorescence (MFI). For the first time demonstrated the existence of different subpopulations CD64+CD16-CD32+CD11b+, CD64+CD16+CD32+CD11b+, CD64-CD16+CD32+CD11b+ Mon in healthy newborn infants and transformation of the phenotype of these subpopulations in deeply premature newborns with the congenital pneumonia varying severity and the respiratory distress syndrome. These features of quantitative and qualitative transformation of 3 subpopulations of Mon were correlated with the severity of congenital pneumonia in deeply premature newborns. The study of the level of the subpopulation CD64-CD16+CD32+CD11b+ Mon can be used as an early diagnostic marker of gravity of inflammatory process in severe congenital pneumonia in deeply premature newborns.