Modeling role of pyruvate in the processes of protein-protein interaction

Using the ABO antibody-Antigen model the influence of natural metabolite pyruvate on the antibody interaction with of erythrocyte antigens, defining their group specificity has been investigated. Before agglutination reaction erythrocytes of A(II)-AB(IV) blood groups, monoclonal anti-A and anti-B antibodies were incubated with sodium pyruvate. Visualization of agglutinates was performed by means of flow cytometry and laser scanning confocal microscopy. Computer-Aided prediction of the spectrum of biological activity of pyruvate by a PASS program proposed major regulatory pathways, in which pyruvate may be involved. It has been demonstrated that pyruvate can regulate the intensity of antigen-Antibody interaction. These results suggest the possibility of using small molecules, for example pyruvate, as molecular probes and prospects of the use of erythrocytes with antigenic determinants of the ABO system expressed on their membranes for studies of protein-protein interactions due to convenient visualization and possibility of quantitative evaluation of this process.

Авторы
Gylmiyarova F.N.1 , Radomskaya V.M.1 , Gusyakova O.A.1 , Ryskina E.A. 2 , Kolotyeva N.A.1 , Shahnovich E.A.1 , Nefedova N.S.1 , Baisheva G.M.1 , Pervova Yu.Y.1 , Pleten A.P.3
Издательство
Russian Academy of Medical Sciences
Номер выпуска
1
Язык
Русский
Страницы
132-140
Статус
Опубликовано
Том
61
Год
2015
Организации
  • 1 Samara state medical university, 89 Chapaevskaya str., Samara, 443099, Russian Federation
  • 2 Peoples' Friendship University of Russia, Moscow, Russian Federation
  • 3 A.I. Evdokimov Moscow State University of Medicine and Dentistry, Moscow, Russian Federation
Ключевые слова
Ab0 antigens; Confocal microscopy; Monoclonal antibodies; Protein-protein interactions; Pyruvate
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/4773/
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