Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins

Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine. © 2016, Springer Science+Business Media New York.

Авторы
Kotelnikova O.V.1 , Zinchenko A.A.1 , Vikhrov A.A.1 , Alliluev A.P. 2 , Serova O.V.1 , Gordeeva E.A.1 , Zhigis L.S.1 , Zueva V.S.1 , Razgulyaeva O.A.1 , Melikhova T.D.1 , Nokel E.A.1 , Drozhzhina E.Y.1 , Rumsh L.D.1
Редакторы
-
Издательство
New York Consultants BureauSpringer / Автономная некоммерческая организация Издательство Российской академии медицинских наук
Номер выпуска
3
Язык
Английский
Страницы
391-394
Статус
Опубликовано
Подразделение
-
Номер
-
Том
161
Год
2016
Организации
  • 1 M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
  • 2 Institute of Medicine, People’s Friendship University of Russia, Moscow, Russian Federation
Ключевые слова
IgA1 protease; immunogenicity; meningococcus; recombinant proteins
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/3888/