On the interaction of ribosomal protein RPL22e with microtubules

Microtubule (MT) protein preparations often contain components of the translation machinery, including ribosome proteins. To understand the biological meaning of it we studied the interaction of ribosomal protein RPL22e with the MT. We found that bacteria expressed purified RPL22e-GFP-6His did co-sediment with brain tubulin MTs with 1.3 µM dissociation coefficient. Such a KD is comparable to some specific MT-associated proteins. Distinct in vitro interaction of RPL22e-GFP with MTs was also observed by TIRF microscopy. In real-time assay, RPL22e-GFP molecules stayed bound to MTs for several seconds, and 15% of them demonstrated random-walk along MTs with diffusion coefficient 0.03 µ2/s. Deletion of basic areas of RPL22e did not have an impact on KD, and deletion of acidic tail slightly increased association with MTs. Interestingly, the deletion of acidic tail increased diffusion coefficient as well. The interaction of RPL22e with MTs is hardly noticeable in vivo in cultured cells, probably since a significant part of the protein is incorporated into the ribosomes. The mobility of ribosomal protein on the MTs probably prevents its interfering with MT-dependent transport and could ameliorate its transport to the nucleus. © 2019 International Federation for Cell Biology

Авторы
Chudinova E.M. 1, 2 , Brodsky I.B. 3 , Nadezhdina E.S.1, 3
Издательство
Wiley-Blackwell Publishing Ltd
Номер выпуска
7
Язык
Английский
Страницы
749-759
Статус
Опубликовано
Том
43
Год
2019
Организации
  • 1 Institute of Protein Research of Russian Academy of Science, Institutskaya str., 4, Pushchino, Moscow Region 142290, Russian Federation
  • 2 Peoples’ Friendship University of Russia (RUDN University), Miklukho-Maklaya str., 6, Moscow, 117198, Russian Federation
  • 3 M.V. Lomonosov Moscow State University, Leninskie Gory, 1-73, Moscow, 119991, Russian Federation
Ключевые слова
co-sedimentation; one-dimensional diffusion; p150Glued; single-molecule imaging; TIRF microscopy
Дата создания
19.07.2019
Дата изменения
19.07.2019
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/38568/