Polyprenyl Phosphates Induce a High Humoral and Cellular Response to Immunization with Recombinant Proteins of the Replicative Complex of the Hepatitis C Virus

The search for new adjuvants remains the critical task for the creation of hepatitis C vaccines due to the weak immunogenicity of biotechnological products. When immunizing mice with the recombinant proteins NS3 and NS5B of the hepatitis C virus (HCV), the adjuvant activity of three immunomodulators was compared. Phosprenyl® on the basis of polyprenyl phosphate (PPP), chemically synthesized analogue of the bacterial cell wall glucosaminyl muramyl dipeptide (GMDP), and IFN-α recombinant protein were tested. GMDP increased the activity of IgG1 antibodies 4–6 times but did not stimulate the production of IFN-γ; IFN-α has not shown any adjuvant properties. The introduction of recombinant HCV proteins together with PPP in low doses increased the activity of IgG2a isotype antibodies 4–7 times and increased IFN-γ secretion 3 times. Thus, it was first shown that PPP polarizes the immune response to Th1-type and is a promising adjuvant for the development of a vaccine against hepatitis C. © 2018, Pleiades Publishing, Ltd.

Авторы
Masalova O.V.1 , Lesnova E.I.1 , Onishchuk A.A. 2 , Ivanova A.M.1 , Gerasimova E.V.1 , Ivanov A.V. 1 , Narovlyansky A.N.1 , Sanin A.V.1 , Pronin A.V.1 , Kushch A.A.1
Номер выпуска
1
Язык
Английский
Страницы
261-263
Статус
Опубликовано
Том
482
Год
2018
Организации
  • 1 Gamaleya National Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, 123098, Russian Federation
  • 2 RUDN, Moscow, 117198, Russian Federation
Дата создания
04.02.2019
Дата изменения
04.02.2019
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/36327/
Поделиться

Другие записи

Marone E.M., Rinaldi L.F., Fedorov S., Sharypova I., Tishchenko A.L.
Italian Journal of Vascular and Endovascular Surgery. Edizioni Minerva Medica. Том 25. 2018. С. 199-202