Different mechanisms of protective and differentiative activities of homological peptides TGENHR and TQVEHR

Previously we identified a six-membered fragment 354TQVEHR 359 of the C-terminal part of the PEDF (Pigment Epithelium-Derived Factor) differentiation factor molecule that shares homology with fragment 41TGENHR46 of the HLDF (Human Leukemia Differentiation Factor) differentiation factor molecule, which is responsible for its differentiation activity. HLDF has been isolated from the culture medium of human promyelocytic leukemia cell line HL-60. Hexapeptides HLDF-6 (TGENHR) and PEDF-6 (TQVEHR) corresponding to these HLDF and PEDF molecule fragments, which were previously shown to induce cell differentiation (Kostanyan et al. (2000) Russian Journal of Bioorganic Chemistry, 26, 505-511), also have neuroprotective properties. Both peptides prevent degeneration of Purkinje cells of rat cerebellar vermis upon chemical hypoxia induced by sodium azide in vivo; this effect is also observed on a behavioral level. Peptide HLDF-6 but not PEDF-6 promotes survival of HL-60 cells upon chemical hypoxia. Peptides HLDF-6 and PEDF-6 affect different second messenger biosynthesis systems in HL-60 cells. HLDF-6 diminishes cyclic AMP level in those cells due to adenylate cyclase inhibition, while PEDF-6 inhibits phosphatidylinositol-specific phospholipase C stimulated by aluminum tetrafluoride anions.

Авторы
Zhokhov S.S.1 , Kostanyan I.A.1 , Gibanova N.V.1 , Surina E.A.1 , Rodionov I.L.2 , Storozheva Z.I.3 , Proshin A.T.3 , Babichenko I.I. 4 , Lipkin V.M.1
Журнал
Номер выпуска
8
Язык
Английский
Страницы
861-869
Статус
Опубликовано
Том
69
Год
2004
Организации
  • 1 Shemyakin/Ovchinnikov Inst. B.C., Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation
  • 2 Branch of Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russian Federation
  • 3 Anokhin Inst. of Normal Physiology, Russian Academy of Medical Sciences, ul. Mokhovaya 14, Moscow 103009, Russian Federation
  • 4 University of People's Friendship, ul. Miklukho-Maklaya 6, Moscow 117198, Russian Federation
Ключевые слова
Adenylate cyclase; Cerebellum; Chemical hypoxia; HL-60 cell line; HLDF; PEDF; Peptide; Phosphatidylinositol-specific phospholipase C; Protective activity
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/3611/