Proteomic analysis of human skeletal muscle (m. vastus lateralis) proteins: Identification of 89 gene expression products

Proteins from bioptates and autoptates of human skeletal muscle m. vastus lateralis were separated by O'Farrell two-dimensional gel electrophoresis (2DE). MALDI-TOF MS and MS/MS enabled identification of 89 protein spots as expression products of 55 genes. A modification of the O'Farrell's method including non-equilibrium electrophoresis in a pH gradient allowed detection - among major sarcomeric, mitochondrial, and cytosolic proteins - of several proteins, such as PDZ- and LIM domain-containing ones (pI > 8.70), fragments of known proteins, and a stable complex of heavy and light ferritin chains. The data underlie further studies of human skeletal muscle proteins in terms of molecular mechanisms of some physiological and pathological processes. © 2009 Pleiades Publishing, Ltd.

Авторы
Kovalyova M.A.1 , Kovalyov L.I.1 , Toropygin I.Y.2 , Shigeev S.V. 3 , Ivanov A.V.1 , Shishkin S.S.1
Журнал
Номер выпуска
11
Язык
Английский
Страницы
1239-1252
Статус
Опубликовано
Том
74
Год
2009
Организации
  • 1 Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, Moscow 119071, Russian Federation
  • 2 Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya ul. 10, Moscow 119121, Russian Federation
  • 3 People's Friendship University of Russia, ul. Miklukho-Maklaya 8, Moscow 117198, Russian Federation
Ключевые слова
Proteins of human skeletal muscle; Proteomics
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/2900/