Isolation and determination of the activity of IgA1 protease from Neisseria meningitidis

A method of the isolation and purification of IgA1 protease from a culture of Neisseria meningitidis serogroup A has been developed. Three inactivated intermediates of the production of the meningococcal vaccine, a culture liquid, as well as a supernatant and precipitate obtained by the precipitation of bacterial cells by cetavlon, served as a starting material. The purity of IgA1 protease was determined by SDS-PAGE. An immunoenzyme assay for determining the IgA1 protease activity has been developed. The yield of the enzyme with a specific activity of 0.5 to 4 million units/mg from 103 g of the cetavlon precipitate (40 l of culture liquid) was about 600 μg. It was shown that IgA1 protease isolated from serogroup A meningococcus is capable of protecting experimental animals (mice) infected with meningococcus of serogroup B. © 2010 Pleiades Publishing, Ltd.

Yagudaeva E.Yu.1 , Zhigis L.S.1 , Razgulyaeva O.A.1 , Zueva V.S.1 , Melnikov E.E.1 , Zubov V.P.1 , Kozlov L.V.2 , Bichucher A.M.2 , Kotel'nikova O.V. 1 , Alliluev A.P. 3 , Avakov A.E.4 , Rumsh L.D.1
Номер выпуска
  • 1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation
  • 2 FGUN Gabrichevskii Moscow Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation
  • 3 Medical Faculty, Russian University of Peoples' Friendship, Moscow, Russian Federation
  • 4 Gabrichevskii Plant for the Production of Bacterial Preparations, FGUN Branch (NPO Microgen), Moscow, Russian Federation
Ключевые слова
Enzymatic activity; IgA1 protease; Immunogenicity; Isolation; Meningococcus
Дата создания
Дата изменения
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