We conducted studies to develop methods of introducing the culture in vitro micropropagation and grown them in the Black Sea city of Sochi. The valuable plant Mentha longifolia (L.) Huds. Apical stem tips (0,2-0,3 mm) were planted on MS basal medium contained of MS salts, 100 mg L-1 myo-inositol, 0.2 mg L-1 thiamine HCl, 0.1 mg L-1 pyridoxine HCl, 0.2 mg L-1 ascorbic acid, 0,5 mg L-1 nicotinic acid, 20 g L-1 sucrose, and 0.6% cell culture reagent agar. Four to five weeks after initial culture, shoots were selected for our regeneration experiment. © 2012 Nova Science Publishers, Inc. All rights reserved.